Fig. 5. Expressions and localizations of a isoforms in oc/oc pancreatic ß-cells. Pancreas (A) and islet (B) total lysates prepared from wild-type, +/oc heterozygous and oc/oc homozygous mice were subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The a subunit isoforms were detected with affinity-purified antibodies against isoforms a1, a2, a3 and a4. The immunoblots obtained with anti-actin and anti-A subunit of V-ATPase antibodies are also shown. The positions of molecular weight markers are indicated (arrowheads). (C) The localization of a subunit isoforms in oc/oc pancreatic ß-cells. Pancreatic ß-cells prepared from wild-type and oc/oc homozygous mice were stained with antibodies against the a isoforms (green) and insulin (red). Merged images are also shown. Bar, 5 µm.