Fig. 6. Dynamics of podoplanin subcellular localization during cell locomotion. Isolated MDCK cells expressing EGFP-tagged PWT, PEC and PCT proteins were imaged by capturing sections of 0.5 µm every 10 minutes for 4 hours. Images in all panels correspond to 3D reconstructions. Arrowheads indicate podoplanin fluorescence signal concentrated at the leading edge of lamellipodial extensions and on the retracting tail, whereas asterisks indicate loss of fluorescence signal on lamellipodial extensions attached to the substratum. The direction of cell migration is indicated by arrows. Animated sequences of these data are shown in supplementary material Movies 3-5.