Fig. 10. Aft1 interacts with both Grx and Trx domains of Grx3 and Grx4, but the Grx domains of Grx3 or Grx4 are responsible for Aft1 transcriptional function and cellular compartmentalisation. (A) Two-hybrid analysis for the following interactions: Aft1 with the Grx domain of Grx3, Aft1 with the Trx domain of Grx3, Aft1 with the Grx domain of Grx4 and Aft1 with the Trx domain of Grx4, determined as in Fig. 2A and Fig. 2C. (B) Northern blot analysis of FET3 and FIT3 expression in samples taken from cultures of the following strains: wild type, grx3grx4, grx3grx4+pMM491(pDomGrx of Grx3) (Dom, domain), grx3grx4+pMM486 (pDomTrx of Grx3), grx3grx4+ pMM506 (pDomGrx of Grx4) and grxgrx4+pMM502 (pDomTrx of Grx4). Cells were exponentially grown in SD at 30°C. (C) GFPAft1 localisation in the grx3grx4 strain co-transformed with pTP20 (pDomGrx, identical results were observed with pTP19) or with pTP17 (pDomTrx, identical results were observed with pTP21) in cultures growing exponentially at 30°C in SD medium.