Fig. 3. Grx3 and Grx4 negatively regulate the expression of FIT3 and FET3 in a manner dependent on Aft1 activity. Cells from the following strains: wild type, grx3, grx4, grx3grx4, aft1 and grx3grx4aft1, were exponentially grown in SD medium plus amino acids, at 30°C, then treated with 2 mM ferrocene. Samples were taken after 4 and 8 hours as indicated. Samples were taken for mRNA isolation and northern blot using FIT3 and FET3 as probes, U2 was detected as a loading control.