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Figure 4


Fig. 4. AFT1 RNA levels are not regulated by Grx3 nor Grx4. Northern blot analysis of AFT1 expression levels in the wild type, grx3grx4 and aft1 mutants and under conditions of overexpression of Grx3, Grx4, or both. Overexpression of Grx3 and Grx4 was driven by the tetO7 or by the ADH1 promoter (pGSTGrx3 or pGSTGrx4) as stated. To regulate gene expression under the tetO7 promoter, we added (+) or not (–) 20 µg/ml doxycycline to the culture media.