Fig. 9. Aft1 nuclear translocation mediated by iron deficit is independent of the Aft1 nuclear localisation mediated by both Grx3 and Grx4. (A) GFPAft1 localisation in the different strains: wild type, grx3grx4 and grx3grx4tetO₇GRX4+pADH1Grx3, upon the addition of 2 mM ferrocene to exponentially growing cells at 30°C in SD medium. (B) Northern blot of FIT3 in mRNA samples extracted from aliquots collected from wild type, grx3grx4 and grx3grx4tetO₇GRX4+pGSTGrx3 upon the addition of 2 mM ferrocene to exponentially growing cells at 30°C in SD medium. (C) Western blot of total protein extracts prepared from the previous experiment. We used anti-HA antibody to detect Grx4 protein and anti-GST antibody to detect Grx3 protein.