Fig. 2. Cell adhesion to ECM substrates. (A) Levels of HTR-8/SVneo cell adhesion to a selected panel of purified ECM proteins. Substrate proteins were coated at 10 µg/ml and cell adhesion was performed in the presence of 1.0 mM Mg²⁺ and 1.0 mM Ca²⁺. Statistical tests were not performed for this descriptive adhesive analysis. (B) Adhesion of HTR-8/SVneo and freshly isolated EVT cells to EMILIN1 and FN. Four experiments were performed and mean ± s.d. reported. No significative difference was seen between EVT and HTR-8/SVneo adhesion capability on EMILIN1 and FN substrates. (C) Perturbation of HTR-8/SVneo cell attachment to gC1q1. Wells were coated with gC1q1 (10 µg/ml). The anti-EMILIN1 mAb 1H2G8 was added to the coated wells just before addition of the cells. In other instances the cells were preincubated with the anti-4 integrin subunit mAb P1H4 for 15 minutes at 37°C and were then allowed to adhere at 37°C for 20 minutes. Data are expressed as mean ± s.d. Post-hoc comparison of control mAb versus test samples after significant ANOVA for repeated-measures main effect, Dunnet's test (n=3). *P<0.01.