Fig. 1. Movement of actin patches visualised in real-time. Cells expressing a GFP-tagged actin-binding protein (Abp1p) were used to visualise the organisation and behaviour of actin patches during the endocytic process (upper panel). Images were recorded for 200 mseconds at 1-second intervals, using a DeltaVision RT Restoration Microscopy System running SoftWoRx^TM image analysis (Applied Precision Instruments, Seattle). Displayed are 12 consecutive images illustrating the arrival of Abp1p at the patch, and its movement and ultimate disassembly from the newly formed vesicle. Arrows indicate the initial arrival of Abp1p at the site and its disassembly and movement at the end of the sequence. Bar, 2 µm.