Fig. 7. Relationship of ROK and ARAP2. (A,B) Effect of ROK on cells with reduced ARAP2 expression. U118 cells were transfected with a plasmid for expression of HA tagged-ROK and ARAP2-siRNA (right panels) and stained using polyclonal anti-HA and either Rhodamine-phalloidin for actin (A) or a monoclonal Ab for paxillin (B). (C) Effect of activated ROK on FA formation in cells with reduced ARAP2 levels. The experiment was similar to that described in B but using GFP-[1-478]ROK instead of full-length ROK. (D,E) Effect of ARAP2 on cells with inhibited ROK activity. U118 cells were transfected with a plasmid for the expression of ARAP2. Twenty-four hours later, the cells were treated with Y27632, a specific ROK inhibitor. The cells were fixed and stained for ARAP2 and either actin (C) or paxillin (D) as indicated. Arrows point to focal contacts. Bars, 10 µm.