Fig. 1. Intracellular localization of FATP4-GFP. (A) Intracellular reticular localization of FATP4 in stably expressing MDCK cells. Shown is a single section obtained by confocal microscopy to demonstrate the reticular distribution clearly. (B) FATP4 colocalization with the ER marker protein CaBP1 in transiently transfected HeLa cells. (C) FATP4 comparison with a plasma membrane marker protein (CD8); transiently expressing HeLa cells. Only one confocal plane close to the bottom is shown. Bars, 10 µm. (D,E) Intracellular localization of FATP4 in terminally polarized MDCK cells. (D) The apical plasma membrane (side view) is stained with an antibody to gp114. (E) The lateral plasma membrane (midsection, one confocal plane only) is identified by p58. Note that FATP4-GFP is present between the plasma membrane and the nucleus, and not colocalizing with p58. Bars, 10 µm.