Fig. 5. A23187 induces the translocation of PKC to the membrane. (A) Cytosolic and membrane fractions were prepared from HEK293 reporter cells treated with vehicle (DMSO) or A23187 (2.5 µM) in the presence or absence of Wnt3a-CM, for western blotting with anti-PKC antibody. The blots were reprobed with anti-actin or anti-MDR antibodies as fractionation controls. (B) The cellular location of PKC in HEK293 reporter cells was determined by immunofluorescence analysis. After fixation, the cells were stained with anti-PKC antibody and observed at 400x magnification. Arrows indicate PKC translocated to the membrane.