Fig. 3. Mid1p is located at the medial cortex before NETO. GFP fluorescence (a,c) and fluostain I staining of the cell wall (b,d) in wild-type cells expressing mid1-4GFP (AP1442) grown at 25°C, or wild-type cells grown for 3 hours in presence (c,d) or absence (a,b) of 11 mM HU to prevent DNA synthesis and to block cells before NETO. mid1-4GFP spots are medially located in monopolarly growing cells after HU treatment (arrowheads), indicating that Mid1p distribution to the medial cortex is established before NETO. Bar, 5 µm.