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Figure 1


Fig. 1. Synthetic cold-sensitive effects with the erv46 deletion. Wild-type (wt, BY4742), erv46{Delta} (SOY01409), vma12{Delta} (SOY01628), vma12{Delta} erv46{Delta} (SOY01654), vma21{Delta} (SOY01629), vma21{Delta} erv46{Delta} (SOY01655), vma22{Delta} (SOY01630), vma22{Delta} erv46{Delta} (SOY01656), vps1{Delta} (SOY01631), vps1{Delta} erv46{Delta} (SOY01657), vps27{Delta} (SOY01632) and vps27{Delta} erv46{Delta} (SOY01658) cells were grown to saturation in YPD medium and adjusted on an OD600 of 3.0. 5 µl of a 10-fold dilution series were spotted onto YPD plates. One set of plates was incubated at 30°C for 2 days and the other at 16°C for 1 week in order to visualize the synthetic growth phenotype of the double mutants. After this long incubation, the slight cold sensitivity of the erv46{Delta} strain is not visible.