Fig. 2. The protective effect of KGF is dependent on de novo protein synthesis. (A) In cytoprotection assays (see legend to Fig. 1A) quiescent HaCaT cells were pre-treated with KGF for different periods of time (1 hour, overnight, or overnight plus 1 hour) or left untreated before the addition of menadione. (B) Results of cytoprotection assays in the absence or presence of the protein biosynthesis inhibitor cycloheximide (CHX) are shown: 2-hour treatment with DMSO before KGF addition (left panel), 2-hour treatment with 10 µg/ml CHX in DMSO before KGF addition (right panel). Results are mean ± s.d. ^*P<0.05 using the Mann-Whitney U test.