Fig. 4. Effect of endogenous ß-catenin on LEF-1-I-mfa interactions. P19 cells were treated with or without Wnt3a for 3 hours. Cells were harvested for cell fractionation and immunoprecipitation with an anti-LEF-1 or anti-Myc antibody (control antibody). Endogenous LEF-1, ß-catenin and I-mfa were detected with their respective antibodies.