Fig. 6. ChlR1 depletion results in mitotic delay and failure. HeLa cells were transfected with siRNA duplexes specific for ChlR1, or GFP as a control. (A) 24 hours following transfection, cells were harvested for western analysis of ChlR1 protein expression using Hel1 antibody. -Tubulin was included as a loading control. Control (B) or ChlR1 siRNA-transfected (C) cells were stained with 1 µM Syto13 to label DNA and filmed by time-lapse imaging using a 20x objective and a TRITC filter set. Numbers in upper left of each panel indicate minutes after start of filming. Bar, 10 µm.