JCS -- Eura et al. 119 (23): 4913 Figure IG5

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 5. Exogenously expressed ${zeta}$ -crystallin does not affect mitochondrial morphology, although it is targeted to the mitochondria. (A) Comparison of amino acid sequences of the GGVG-motif-containing regions of MIB and ${zeta}$ -crystallin. (B) Mitochondrial morphology of HeLa cells expressing ${zeta}$ -crystallin-FLAG. Intracellular localization of ${zeta}$ -crystallin was analyzed by immunofluorescence microscopy. Mitochondria were counterstained with MitoTracker. Details of the boxed regions in top panels are shown in the bottom panels. Bars, 10 µm. (C) Expression levels of MIB-FLAG and ${zeta}$ -crystallin-FLAG in HeLa cells were examined by SDS-PAGE and subsequent immunoblotting with anti-FLAG IgG. (D) Cells with the indicated mitochondrial morphology shown in B were quantified. At least 100 cells were counted in at least three distinct optical fields. Control: empty vector. (E) Binding of ${zeta}$ -crystallin-FLAG to Mfn1 as examined by MBP-Mfn1 ${Delta}$ C-beads. HeLa cell extracts expressing ${zeta}$ -crystallin-FLAG or MIB-FLAG were incubated with MBP- or MBP-Mfn1 ${Delta}$ C-conjugated beads. Other conditions were as described in Fig. 1D.