Fig. 5. Ca²⁺ exchange between mitochondria and ER. ER vesicles and mitochondria were labelled with Mag-Fura-2 and Rhod-2, respectively. KCl (45 mM) (A), hypoxia (B), 100 µM t-ACPD (C), 1 µM thapsigargin (D), 1 µM CCCP (E) and 3 µM Taxol (F) were applied as indicated. The traces were obtained from fluorescence changes in the areas that contained both ER vesicles and mitochondria. The data are representative of at least five trials for each protocol performed in neurons from three different cell batches.