Fig. 5. Accumulation of splice variants of perlecan in tsTM18 cells during culture at 39°C. Incubation times at 39°C are indicated above the gel. A 1661-bp RT-PCR product was predicted on the basis of the sequence. This product is present in CHO-K1 cells and in tsTM18 cells incubated at 34°C. However, during culture of tsTM18 at 39°C, the amount of the 1661-bp product decreases gradually, and a number of different splice variants appear.