Fig. 3. Rrm4G is assembled into cytoplasmic particles that shuttle along cytoskeletal tracks. (A) AB33rrm4G filaments are shown 4 hours after induction. Epifluorescence images are taken from time-lapse Movie 1 in supplementary material. Rectangles indicate regions that are magnified below. Arrows indicate particles that move along cytoskeletal tracks at the distal pole (left panels) and at the tip (right panels; time in seconds). Bars, 5 µm. (B) Whisker diagram indicating density of Rrm4G-containing particles (particles/µm) in strain AB33rrm4G measured at the onset or after 6 hours of filamentation. The top and bottom of each rectangle represent the 75th and 25th percentiles, respectively. The median is the horizontal line within the box, and the top and bottom borders of the whiskers show the 95th and 5th percentiles, respectively. More than 200 particles were analysed for each strain. The number of the transformant is given below. (C) Whisker diagram (labelling as in B) showing the relative fluorescence intensity of Rrm4G-containing particles in strain AB33rrm4G measured at the onset or after 6 hours of filamentation. More than 80 particles each were analysed (number of transformant is indicated below).