Fig. 5. Rrm4-containing particles shuttle in vivo along microtubules. (A) Filaments of AB33rrm4G were treated with benomyl 4 hours after filament induction and time-lapse movies were taken at the onset (0 seconds, top) and after 23 minutes (bottom) of treatment. Overlays of two frames 1 second apart (coloured in green and red) from supplementary material Movie 4 are shown (moving particles in green and red, top; non-motile particles in yellow, bottom). Bar, 10 µm. (B) In vivo co-localisation of Rrm4R-containing particles (red, arrows) with dynamic Tub1G-decorated microtubules (green) in AB33rrm4R/tub1G filaments 4 hours after induction. Bar, 5 µm. Frames are from supplementary material Movie 5. Rectangles indicate regions that are magnified below. Bar, 1 µm. Time is indicated in seconds.