JCS -- Jeon et al. 119 (23): 4994 Figure IG3

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Fig. 3. Effects of SPC and TGF-ß3 on the transcriptional levels of smooth-muscle-specific markers. (A) Serum-starved hATSCs were treated with vehicles, 2 µM D-erythro-SPC or 2 ng/ml TGF-ß3 for the indicated time, and ${alpha}$ -SMA mRNA was quantified by real time RT-PCR analysis. Data represent average values ± s.e.m. of triplicate determinants. ^*Significantly different from control value (P<0.05). (B) Serum-starved hATSCs were exposed to 2 µM D-erythro-SPC or 2 ng/ml TGF-ß3 for 24 hours, and the levels of ${alpha}$ -SMA, h₁-calponin, SM22 ${alpha}$ and GAPDH mRNA were determined by semi-quantitative RT-PCR using gene-specific primers. Representative data from three independent experiments are shown. (C) hATSCs were treated with vehicles, 2 µM D-erythro-SPC or 2 ng/ml TGF-ß3 for 24 hours, and the expression levels of h₁-calponin and SM22 ${alpha}$ were quantified by real time RT-PCR. Data represent average values ± s.e.m. of triplicate determinants. ^*Significantly different from control value (P<0.05).