JCS -- Jeon et al. 119 (23): 4994 Figure IG4

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Fig. 4. Effects of SPC and TGF-ß3 on the formation of actin stress fibers and the localization of ${alpha}$ -SMA in hATSCs. Serum-starved hATSCs were treated with vehicles, 2 µM D-erythro-SPC or 2 ng/ml TGF-ß3 for 4 days, and immunostaining performed. ${alpha}$ -SMA and F-actin were double-stained with anti- ${alpha}$ -SMA and Alexa Fluor 568 phalloidin, and analysed using a Leica TCS-SP2 laser scanning confocal microscope (Leica Microsystems, Germany) with a magnification of 400x. The overlaid images of the double staining are shown. Representatives of three independent experiments are shown.