Fig. 4. Alignment of KASH domains from various proteins. The amino acid residues present in the predicted transmembrane regions are enclosed by red lines. The Zyg-12B KASH domain sequence is used because the Zyg-12C KASH domain sequence in the database lacks 16 residues encompassing part of the neck and transmembrane regions. Dark green, conserved amino acids; light green, amino acids with similar chemical properties in more than half of the KASH domains. Mm, Mus musculus; Hs, Homo sapiens.