(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Sister chromatid cohesion defects in Xenopus egg extracts depleted of cohesin. (A) Upper panel. Western blot of untreated total (total extract) and IgG-depleted ({Delta}Mock) Xenopus egg extract, and Xenopus egg extract depleted by antibodies against XSmc1 and XRad21 ({Delta}Cohesin). The blot was probed with an affinity-purified XSmc1 antibody that recognizes a single band of 155 kDa (present in total extract and {Delta}Mock), which is absent in cohesin-depleted extract ({Delta}Cohesin). Lower panel. The same blot probed with an affinity-purified antibody against the XCAP-G condensin subunit, recognizing a single band of 130 kDa (present in control, IgG- and cohesin-depleted extracts. (B) Immunofluorescence images of sperm chromosomes assembled in mock- and cohesin-depleted extracts that had been cycled through interphase to allow DNA replication, then back into mitosis. Chromosomes were spun down onto coverslips 60 minutes after induction of mitosis and processed for immunofluorescence using an antibody that recognizes a subunit of the condensin complex, XCAP-G (blue) and the kinetochore protein BubR1 (red). Bar, 10 µm.