Fig. 3. Examples of anaphase chromosome segregation defects observed in the absence of cohesin; mock- and cohesin-depleted extracts containing spindles fixed 15 minutes after anaphase induction. Note that, chromosomes clearly segregated in three out of four control spindles, whereas cohesin-depletion causes segregation failure and/or unequal distribution of chromatids. Microtubules are red and chromosomes are blue-green.