Fig. 1. Subcellular location and dynamics of GP
1-YFP and its lipid motif mutants in cowpea protoplasts. Protoplasts expressing (A) GP
1-YFP, (B) GP
1(G2A)-YFP, (C) GP
1(C5S)-YFP, (D) GP
1(G2A/C5S)-YFP, (E) unfused YFP. N, nucleus; V, vacuole. (F) FRAP curves of YFP (triangles); GP
1-YFP (open diamonds); GP
1(G2A)-YFP (circles), GP
1(C5S)-YFP (squares) and GP
1(G2A/C5S)-YFP (filled diamonds). Bars, 10 µm.