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Figure 1


Fig. 1. Subcellular location and dynamics of GP{alpha}1-YFP and its lipid motif mutants in cowpea protoplasts. Protoplasts expressing (A) GP{alpha}1-YFP, (B) GP{alpha}1(G2A)-YFP, (C) GP{alpha}1(C5S)-YFP, (D) GP{alpha}1(G2A/C5S)-YFP, (E) unfused YFP. N, nucleus; V, vacuole. (F) FRAP curves of YFP (triangles); GP{alpha}1-YFP (open diamonds); GP{alpha}1(G2A)-YFP (circles), GP{alpha}1(C5S)-YFP (squares) and GP{alpha}1(G2A/C5S)-YFP (filled diamonds). Bars, 10 µm.