Fig. 1. Localisation of symplekin and ZONAB in epithelial cells. HT29-16E cells (A,B), wild-type MDCK cells (C,D), or MDCK cells stably transfected with a cDNA construct encoding flag-tagged full-length symplekin (E), were fixed and permeabilised and then stained with either anti-ZONAB and symplekin antibodies (A-D) or anti-flag antibodies (E). A, C, D and E are epifluorescence images and B shows images taken with a confocal microscope. Note, junctional staining for symplekin was often absent (C) and only observed occasionally (D) in MDCK cells.