Fig. 5. Localisation of symplekin and ZONAB in HT29-16E cells. Control and symplekin-depleted HT29-16E cells were fixed and stained for symplekin and ZONAB. Note that in non-depleted cells, much of the stainings for both proteins was nuclear. In depleted cells, ZONAB was primarily in the cytosol and there was only a small amount of nuclear and junctional staining.