Fig. 2. Northern blot analysis of the tissue distribution of PZDRN3 mRNA. A nylon membrane containing fractionated polyadenylated RNA from adult human tissues was subjected to hybridization with a [³²P]-labeled human PDZRN3 cDNA probe. The arrowhead indicates a hybridizing 5.5 kb mRNA. The blot was also probed for ß-actin mRNA as a loading control. The positions of molecular size standards are indicated on the left. Br, brain; H, heart; Sk, skeletal muscle; Co, colon; Th, thymus; Sp, spleen; K, kidney; Li, liver; In, small intestine; Pl, placenta; Lu, lung; Le, peripheral blood leukocytes.