Fig. 5. Up-regulation of PDZRN3 during differentiation of C2C12 myoblasts into myotubes. (A) C2C12 myoblasts induced to differentiate by culture for the indicated times in DM were both examined by phase-contrast microscopy (upper panels) and subjected to immunofluorescence analysis with anti-MHC (lower panels). Bar, 25 µm. (B) C2C12 cell lysates prepared at the indicated times after induction of differentiation were subjected to immunoblot analysis with antibodies to the indicated proteins. (C) The amount of each protein was quantified by image analysis of blots similar to that shown in (B), normalized by the amount of -tubulin and expressed as a percentage of the maximum normalized value in each experiment. Data are means ± s.e.m. from four independent experiments.