Fig. 6. Inhibition of myoblast differentiation by depletion of PDZRN3. (A) C2C12 cells were transfected with a control siRNA or an siRNA specific for PDZRN3 mRNA and were induced to differentiate by culture for 4 days in DM. The cells were then examined by phase-contrast microscopy (upper panels) and subjected to immunofluorescence analysis with anti-MHC (lower panels). Bar, 25 µm. (B) Lysates of cells treated as in (A) and cultured in DM for the indicated times were subjected to immunoblot analysis with antibodies to the indicated proteins.