Fig. 7. Lack of effect of PDZRN3 depletion on expression of myogenin. (A) C2C12 cells were transfected or not (Mock) with a control siRNA or an siRNA specific for PDZRN3 mRNA and were induced to differentiate by culture for 5 days in DM. They were then examined by phase-contrast microscopy (left panels) and subjected to immunofluorescence analysis with anti-myogenin (middle panels). Nuclei were also stained with 4',6-diamidino-2-phenylindole (DAPI, right panels). Bar, 25 µm. (B) The percentage of DAPI-stained nuclei that were positive for myogenin was determined in each of 10 randomly chosen fields (magnification, 200x) for cells treated as in (A). Data are means ± s.e.m. from a representative experiment.