Fig. 8. Expression of PDZRN3 during regeneration of damaged mouse skeletal muscle. (A) Muscle injury and regeneration were induced by intramuscular injection of cardiotoxin in the hind limb and sections of muscle isolated before or 1, 3, 5, or 9 days after injury were stained with hematoxylin-eosin. Bar, 50 µm. (B) Homogenates of muscle isolated at the indicated times after injury were subjected to immunoblot analysis with antibodies to the indicated proteins. The abundance of PDZRN3 was quantified by image analysis of blots similar to that shown in the upper panel and expressed as a percentage of the value at 5 days after injury (lower panel); data are means ± s.e.m. from three independent experiments. (C) A section of muscle at 5 days after injury was double-stained with anti-PDZRN3 (green) and anti-myogenin (red). Myogenin-positive nuclei are indicated by arrows. Myogenin-negative nuclei in myotubes are indicated by arrowheads. Bar, 25 µm.