Fig. 4. Tissue and subcellular distributions of RSL1D1 overlap with those of nucleostemin. (A,B) Northern blot analyses of expression patterns of RSL1D1 and nucleostemin in the developing whole embryos from E10.5 to E16.5 (A) and in the adult mice (B). (C1-E3) Double-labeled immunofluorescence and confocal analyses showing colocalization of endogenous nucleostemin (NS, red) and Myc-tagged RSL1D1 (green) (C), NS (red) and B23 (green) (D), NS (red) and fibrillarin (Fib, green) (E). High magnifications of the indicated areas (squares) are shown in (C2, D2 and E2). Dashed lines delineate the nucleo-cytoplasmic boundaries. Bars, 5 µm for C1, D1, E1; 2 µm for C2, D2 and E2. Quantification of colocalization is shown in C3, D3 and E3. All pixels were plotted on the basis of their red (y-axis) and green (x-axis) fluorescence intensities, and pseudocolored on the basis of the event frequency, with red representing the highest and blue the lowest event frequency.