Fig. 6. Downregulation of periplakin and keratin 8 expression by siRNA transfections. (A) western blotting of total cell extracts from transient transfections with control or specific siRNAs. Total cell extracts were collected 72 hours after transfection and 20 µg protein aliquots were blotted with antibodies against indicated proteins. (B) Immunofluorescence staining of MCF-7 cells transfected with scrambled control siRNA (top row), Periplakin siRNA (middle row) or K8 siRNA (bottom row) showing K8 staining (red channel on the left) Periplakin staining (green channel, middle column) and merged image (on the right). (C) Control and K=8 siRNA transfected cells stained with Desmoplakin antibody (green channel, on the left) and merged with K=8 antibody staining (red channel). Scale bar is 20 µm in both B and C.