Fig. 6. Projection images of the inner ear reveal morphological distinctions between control and glo mutant embryos. Whole-mount embryos were stained with Texas-Red-conjugated phalloidin and image volumes were acquired by two-photon microscopy. Image projections were made from each volume that encompassed the region of the lateral, rostral and caudal epithelial projections into the otic vesicle. Each ear was classified by extent of epithelial projection formation and fusion (B, see Materials and methods for description of classification scheme). Control embryos (n=33) all were either category 1 or 2, with fully formed projections and fusion of at least the rostral and lateral epithelial pillars. glo mutant embryos (n=38) were spread across categories 2-5, with most lacking any fusion of epithelial pillars (A). Wild-type and glo mutant morphology were statistically different (P=8.6x10^-9, df=4, Chi Square=43.39). Bar, 50 µm.