Fig. 1. Multiple genome-wide screens identify common genes required for tolerance to replication stress. (A) Gene deletion strains hypersensitive to hydroxyurea (HU) identified by the approaches of Bennett et al., Hartman and Tippery, Parsons et al. and this study were compiled to identify the shared HU sensitive strains (Bennett et al., 2001; Hartman and Tippery, 2004; Parsons et al., 2004). Number of gene deletions shared between datasets (intersections) or unique to each individual dataset (peripheries) are indicated. (B) HU-sensitive gene deletion mutants identified by at least three of the four approaches were classified by Gene Ontology (GO) process according to the Biological General Repository for Interaction Datasets (BioGRID; www.thebiogrid.org) (Stark et al., 2006). The network was created with Osprey visualization software (Breitkreutz et al., 2003).