JCS -- Woolstencroft et al. 119 (24): 5178 Figure IG5

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 5. Deletion of CRT1 or PBP1 suppresses the HU sensitivity of a ccr4 ${Delta}$ strain. (A) ccr4 ${Delta}$ crt1 ${Delta}$ (MT3771), ccr4 ${Delta}$ pbp1 ${Delta}$ (MT3777), wild-type (BY4741), ccr4 ${Delta}$ (MT3768), crt1 ${Delta}$ (MT3770) and pbp1 ${Delta}$ (MT3776) strains were tested for HU sensitivity by serial dilution analysis, as described in Fig. 2. (B) Indicated strains were assessed for irreversible HU sensitivity using a spiral plating system, as described in Fig. 2C. (C) Rad53 activity and phosphorylation were assessed in ccr4 ${Delta}$ dun1 ${Delta}$ (MT3772), ccr4 ${Delta}$ dun1 ${Delta}$ crt1 ${Delta}$ (RSYS07) and wild-type (BY4741) strains after exposure to 0.2 M HU for 3 hours, as described for Fig. 3.