Fig. 6. Expression of TGF-ß1. (A-J) Immunohistochemistry for TGF-ß1 at wound sites treated with control sODN (A-E) and Cx43-asODN (F-J). Bars, 200 µm (A,F) and 50 µm (B-E,G-J). Black arrows show the nascent edge of the epidermis. TGF-ß1 staining is considerably stronger in the epidermis of wounds treated with Cx43-asODN (I,J) compared with those treated with control sODN (D,E). Red and black arrowheads indicate representative TGF-ß1-elongated fibroblast-like cells and rounded presumptive leukocytes, respectively. (K) Real-time PCR analysis of the expression on days 1, 2 and 7 (n=5 for each) of mRNA for TGF-ß1 at wound sites treated with control sODN (white bars) or Cx43-asODN (black bars). Data are expressed as the mean ± s.e.m.; ^*P<0.05.