Fig. 7. Granulation tissue formation and fibroblast migration. (A,B) Fibroblast-like cell recruitment into skin wounds treated with control sODN (A) and Cx43-asODN (B), analyzed using TRITC-phalloidin and DAPI nuclear staining on day 2. (C) Number of fibroblast-like cells at each wound site per field of view for wounds treated with control sODN (white bars, n=5) or Cx43-asODN (black bars, n=5). (D-E) Images of wounds in fibroblast cultures at (D) the time of wounding and (E) 4 hours after wounding. (F) Wound-healing assay of fibroblast migration shows that migration is significantly faster after treatment with Cx43-asODNs. (G,H) Staining of fibroblasts for Cx43 in cultures treated with (G) control and (H) Cx43-asODN after 2.5 hours. (I) Quantification of Cx43 expression 2.5 hours after Cx43-asODN treatment, expressed as a percentage of control (n=8 per time point). Data are expressed as the mean ± s.e.m.; ^*P<0.02, ^**P<0.01, ^***P<0.0001. Bars, 50 µm. For a high-resolution figure, please see the online version of this article.