Fig. 5. Protein velocity maps for CHO K1 cells co-transfected with an adhesion protein-EGFP and actin-mRFP and plated on 2 µg/ml fibronectin. (A) Velocity maps in protruding regions of the cell for EGFP conjugated with 5-integrin (96 frames analysed), paxillin (51 frames analysed), FAK (50 frames analysed), talin (55 frames analysed), vinculin (30 frames analysed), -actinin (100 frames analysed) and the corresponding actin-mRFP velocity maps. The color coding for the velocity vectors is universal with blue being slow velocities and red being faster velocities. The scale of the velocity vectors is different for each plot and is referenced on each image with a 0.5 µm/minute velocity scale arrow. Spatial scale bars are 5 µm. (B) Plot of the median relative magnitude and directional correlation coefficient scores relative to actin for each protein in CHO cells. Pixel size of 0.215 µm. Error bars give the s.d.