Fig. 7. Asymmetric localization of polarity proteins. Embryos, anterior left. (A) DE-cadherin (green), P-Tyr (red). (B) Arm (green), F-actin (red). (A,B) Neither DE-cadherin nor Arm localize to denticles (white arrows). Cortical Arm levels are higher in denticle-producing cells (brackets). Arm levels are somewhat higher at dorsal/ventral cell borders (blue arrowheads) than at anterior/posterior cell borders (red arrows). (C-E) Fmi (single channel or green). (C) Prior to denticle development Fmi localizes uniformly to cortex. (D,E) F-actin (red), Fmi (green), Arm (violet). Cells just anterior to denticle belts accumulate less Fmi (bracket). Cells just posterior to denticle belts accumulate Fmi uniformly on all cell interfaces (arrows). (E) Close-up, yellow-boxed region in D. Denticle-secreting cells accumulate more Fmi at anterior/posterior margins (red arrows) than on dorsal/ventral margins (blue arrowheads). (F) Stills, movie of Fz-GFP. Times in hours:minutes:seconds. (G) False-color closeup of F; pixel intensity color-coded from blue to yellow. (H-K) Still images, Dsh-GFP. Red arrows – asymmetric accumulation at anterior/posterior boundaries. (F-K) Blue arrowheads=presumptive vesicles, red arrows/arrowheads=asymmetric accumulation along anterior-posterior boundaries, red brackets=presumptive denticle belts, blue arrows=presumed actin condensations. Scale bars, 5 µm.