Fig. 4. Antibody-induced redistribution of 6ß1 integrin in wild-type (WT) and CD9^–/– oocytes. 6ß1 integrins were aggregated at the surface of WT and CD9^–/– ZP intact eggs using a combination of anti-integrin 6 and goat anti-mouse antibodies and its distribution was studied by confocal microscopy as described in the Materials and Methods. As a control, wild-type eggs that were fixed before labelling were analysed. Note that the 6ß1 integrin patches were bigger and more heterogeneous in CD9-null oocytes than in WT oocytes. Bar, 40 µm.