Fig. 2. Three dimensional reconstruction of PtdIns(3,4,5)P₃ distribution at the open phagocytic cup. The image series is reconstructed from 20 confocal optical slices through a living cell during phagocytosis, where green shows the high-intensity GFP-Akt-PH binding regions. The reconstruction (left-hand panel) shows an incomplete phagosome that has been sliced through to reveal the inner surface of the open phagocytic cup. The position of the zymosan particle shown in the diagram is also shown in image a. (b) The same data without the zymosan particle. (c and d) The same phagocytic cup reoriented and enlarged to permit visualisation of the inner surfaces of the phagocytic cup. The complete rotation of the 3D reconstruction is shown in Movie 2 in the supplementary material. Bars, 2 µm.