Fig. 3. Distribution of DiI and GFP-Akt-PH. (a) The localisation of exogenously loaded DiIC₁₆(3) to the uropod of a neutrophil. The insert shows the distribution of DiIC₁₆(3) intensity at the membrane in the region of the uropod. (b) The distribution of GFP-Akt-PH (green) and DiIC₁₆(3) (red) in a polarised neutrophilic HL60 cell. (c) The distribution of DiIC₁₆(3) in a neutrophil undergoing phagocytosis. The complete data set for this is shown in Movie 3 of supplementary material. (d) The distribution of GFP-Akt-PH (green) and DiIC₁₆(3) (red) in a neutrophilic HL60 cell during phagocytosis, together with a plot of the intensities of the two probes around the cell periphery and unclosed phagosome. (e) Phase-contrast and confocal fluorescent images of neutrophilic HL60 cells expressing GFP-Akt-PH after treatment with LY294002 (50 µM, 15 minutes, 37°C), showing failure to complete phagocytosis of several iC3b-opsonised zymosan particles and the lack of GFP-Akt-PH translocation to the phagocytic cups (indicated in the phase-contrast image). Bars, 5 µm.