Fig. 1. Schematic diagram of the MKLP1 coding sequence and its deletion constructs. The first diagram shows the approximate functional domains of MKLP1, represented on the scale of MKLP1 1-856. Shown below are the diagrams of the constructs used in this study. Numbers on the right denote the numbers of amino acids in the mutation constructs; construct D456-710 has 254 aa deleted (from 456-710) compared with MKLP1 1-856. The enhanced green fluorescent protein (eGFP) tag fused to the N-terminus is shown in green.