Fig. 3. Distribution of eGFP-tagged MKLP1(1-856) or MKLP1(1-840) in HEK293A cells. (Upper panels) eGFP-MKLP1(1-856) concentrated in the mid-body at the end of cytokinesis and associated with microtubules when expressed in HEK293A cells. Arrowheads indicate mid-body. (Lower panels) eGFP-MKLP1(1-840) bound easier to microtubules and accumulated at the periphery of the cell where the plus ends of microtubules were located. Arrowheads indicate accumulated eGFP. DNA in the nucleus was stained with Hoechst 33258 dye (blue). Bar, 25 µm.