Fig. 5. Localization of MKLP1-deletion constructs containing N-terminal domains that had been transfected into hippocampal neurons. Arrowheads indicate the location of axons. Immunofluorescence microscopy images of cells expressing MKLP1-deletion mutants (GFP, green); MAP2 immunoreactivity in the same neurons (MAP2, red). Deletion constructs MKLP1(1-456), -(1-710) and -(1-840) showed a different dendritic localization than full-length MKLP1. Bar, 25 µm.