Fig. 7. Influence of MVP on cell surface marker protein expression. (A) Expression of CD13 (detected by two different antibodies Ab1, Ab2) and CD36 in a representative MVP-expressing (thick line) as compared with a vector control H65 cell clone (thin line) was measured by FACS analysis. (B) Presence of ICAM-1 on the surface of two MVP-expressing (pMVP) and control (pSport6) H65 clones each was determined by FACS analysis and compared with the integrins vß5 and vß3. Thick and thin lines represent probes with specific primary and unspecific control antibodies, respectively. (C) Basal and IFN--induced ICAM-1 expression of stably MVP-transfected (pMVP) and vector control (pSport6) H65 cell clones was analysed by western blotting. (D) The presence of ICAM-1 mRNA was determined by RT-PCR in H65 cell clones with and without ectopic MVP expression. (E) Human U373 glioblastoma cells were infected with adenoviruses expressing shRNA targeting human MVP or Renilla luciferase as a non-expressed control for 5 days and treated with 250 IU/ml IFN- for 24 hours. Whole cell lysates were analysed by western blot and probed with the indicated antibodies. All experiments were performed at least twice with comparable results.